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å¤æ—¦å¤§å­¦ä¸å¾ä¸œæ•™æŽˆè¯¾é¢˜ç»„《Bioact. Mater.》：准三¾l´ç»†èƒžç ”½I¶åã^å°çš„研制åŠç»†èƒžè¡Œä¸ºç ”½I?/span>
                  
						2022-01-27     æ¥æº:中国èšåˆç‰©ç½‘  点击‹Æ?/span>
                  
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                  RFP-hMSC¾l†èƒžåœ¨å…·æœ‰ä¸åŒæ‹“扑åŞ貌表é¢çš„轮廓速度ä¸?/span>V拉äŽ×= 10 ?m h-1 (S)ã€?/span>29 ?m h-1 (M)ã€?/span>24 ?m h-1 (L)ã€?/span>76 ?m h -1 (F) å’?/span>Véžæ‹‰ä¼?/span>= 14 ?m h-1 (S)ã€?/span>33 ?m h -1 (M)ã€?/span>17 ?m h-1 (L)ã€?/span>35 ?m h-1 (F)。在循环拉äŽ×下细胞迿UÀL˜¾½Cºå‡ºå¾ˆå¼ºçš„æ–¹å‘性ã€?/span>通过计算MSDçš?/span>Xå’?/span>Y分é‡åQ?/span>MSD Xå’?/span>MSD Y åQ‰ï¼Œòq¶å°† MSD Y / MSD X定义为方å‘性，数å€?/span>ä¸?/span> 1.4 (S)ã€?/span>5.1 (M)ã€?/span>3.8 (L) å’?17.2 (F)ã€?/span>  
				
                  
					 
				
                  
				
                  
					
                  
				
                  
å›?/span>7. 循环拉äŽ× (St.) å’Œéžæ‹‰äŽ× (N.St.) 下å•ä¸ªç»†èƒžçš„跟踪路径ã€?/span>图中昄¡¤ºäº†æ¯ä¸ªåŸºæ¿çš„30个细胞轨˜qV€?/span>MSD是根æ®æ¯1ž®æ—¶˜q移轨迹的端到端è·ç¦»è®¡ç®—得出的ã€?/span>MSDçš?/span>Xå’?/span>Y分é‡ç”¨äºŽè®¡ç®—˜q移的方å‘æ€?/span>ã€?/span>
                  
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å›?/span>8. å…Þpšç„¦æ˜¾å¾®é•œZ轴层扫的三维å åŠ å›¾ã€‚其中ç°è‰²è¡¨½Cºæ料；¾U¢è‰²è¡¨ç¤ºå¾®ä¸åQ›ç»¿è‰²è¡¨½Cºé»ç€æ–‘蛋白；è“色表示¾l†èƒžæ ¸ã€‚此处，â€?Dâ€?/span>表示òq›_¦ PDMS 表é¢ä¸Šçš„¾l†èƒžåQ?/span>â€?Dâ€?/span>表示åŸø™´¨èƒ¶ä¸­çš„细胞，â€?/span>quasi-3Dâ€?/span>表示è¢?/span>PDMS微柱包围的微井中的细胞，其中“Sâ€?/span>ã€?/span>“Mâ€?/span>å’?/span>“Lâ€?/span>表示ž®ã€ä¸­ã€å¤§å¾®äº•ã€?/span> ¾l†èƒžåœ?/span>二维和准三维中培å…?/span>4 håQŒåœ¨ä¸‰ç»´ä¸­åŸ¹å…?/span>24 hã€?/span>  ä¸ÞZº†è€ƒå¯Ÿä¸åŒ¾l†èƒžå¯¹äºŽä¸‰ç§å¾®çŽ¯å¢ƒçš„å“应åQŒå°†ä¸‰ç§¾l†èƒžåœ¨äºŒ¾l´ã€å‡†ä¸‰ç»´å’Œä¸‰¾l´å¾®çŽ¯å¢ƒä¸?/span>的细胞å“应。这三秾l†èƒžåˆ†åˆ«ä¸?/span>hMSCåQˆäh骨髓间充质干¾l†èƒžåQ?/span>ã€?/span>HFFåQˆäh包皮æˆçº¤¾l´ç»†èƒžï¼‰ã€?/span>HUVECåQˆähè„é™è„‰å†…皮细胞）。需è¦å¼ºè°ƒçš„是，˜q™é‡Œòq?/span>没有动用循环拉äŽ×。图9A所½Cºï¼Œ¾l†èƒžåœ¨äºŒ¾l?/span>微环境中4 hå†?/span>ž®±é“ºå±?/span>良好。但在三¾l?/span>微环å¢?/span>ä¸?/span>ž®½ç®¡åŸ¹å…»24 håQŒç»†èƒ?/span>ä»ç„¶åœ¨åŸºè´¨èƒ¶ä¸­è¶‹äºŽåœ†å½¢ã€‚在准三¾l?/span>微环å¢?/span>中，¾l†èƒžè¡¨çŽ°å‡ÞZ¸åŒçš„å½¢æ€ï¼šM使细胞比Så’?/span>L膜更圆。图9B昄¡¤ºäº†å®šé‡åˆ†æžçš„¾l“果。圆度确实是二维<å‡?/span>三维<三维。此外，æ¯ä¸ª¾l†èƒžä¸?/span>é»ç€æ–‘蛋ç™?/span>的积分强度äؓ二维: 准三¾l?/span>:三维= 5:3:1。因此，准三¾l´å¾®çŽ¯å¢ƒä»‹äºŽäºŒç»´å’Œä¸‰¾l´ä¹‹é—ß_¼Œä¸?/span>相对更接˜q‘三¾l´ã€‚在准三¾lß_¼ˆSã€?/span>M å’?LåQ‰ä¸­åQ?/span>M¾l„在所有三¾cÈ»†èƒžçš„三个斚w¢åQ?/span>å¾®ä¸ã€åœ†åº¦å’Œ¾l†èƒžé¢ç§¯åQ‰å‡æ˜„¡¤ºå‡ºæœ€é«˜å€¹{€?/span>  
			
                  


                  
				
                  

                  
			
                  


                  
å›?/span>9. 在二¾lß_¼ˆPDMSòq³é¢ä¸Šï¼‰ã€å‡†ä¸‰ç»´åQ?/span>PDMS 微井中）和三¾lß_¼ˆåŸø™´¨èƒ¶ä¸­åQ‰å¾®çŽ¯å¢ƒä¸­åŸ¹å…Èš„ä¸åŒ¾cÕdž‹çš„细胞ã€?/span>(A) 染色çš?/span>hMSCã€?/span>HFFå’?/span>HUVEC çš„è§å…‰æ˜¾å¾®ç…§ç‰‡ï¼Œå…¶ä¸­å¾®ä¸ä¸ºçº¢è‰ÔŒ¼Œé»ç€æ–‘蛋白äØ“¾l¿è‰²åQŒç»†èƒžæ ¸ä¸ø™“艌Ӏ?/span>(B)二维和准三维¾l†èƒžåŸ¹å…» 4 håQŒä¸‰¾l´ç»†èƒžåŸ¹å…?/span> 24 hå?/span>å¾®ä¸å’Œé»ç€æ–‘蛋白的¿U¯åˆ†å¼ºåº¦å’Œç»†èƒžé»é™„å‚æ•°çš„¾lŸè®¡¾l“æžœã€?/span>
                  
                  通过以上研究å‘现åQŒç»†èƒ?/span>在中½{‰å¾®äº?/span>åQ?/span>MåQ?/span>ä¸?/span>çš„å–å‘ã€é“ºå±•ã€?/span>˜q移å‡æ¯”ž®å¾®äº?/span> (S) 和大微井 (L)é«?/span>。由于细胞çƒåº¦çš„™åºåºä¸?/span> M > L > SåQŒç ”½I¶äh员推‹¹‹å‡†ä¸‰ç»´¾l†èƒžé»é™„改å˜äº†ç»†èƒžè¡Œä¸ºã€‚也ž®±æ˜¯è¯ß_¼ŒMå¯èƒ½å……当â€?/span>é€?/span>åº?/span>支架â€?/span>æ?/span>帮助¾l†èƒžé»é™„ã€é‡æ–°å®šå‘å’Œ˜q移。äؓ了进一步é˜æ˜?/span>â€?/span>é€?/span>åº?/span>支架â€?/span>˜q™ä¸€æ¦‚念åQŒç ”½I¶äh员将三秾l†èƒžç”¨èƒ°é…¶æ¶ˆåŒ–之åŽé‡æ‚¬äºŽ¾l„织培养æ?/span> (TCP) 表é¢20 分钟˜q›è¡Œæ‹æ‘„åQŒèŽ·å¾—清晰的¾l†èƒžæ˜‘Ö¾®é•œç…§ç‰‡ã€‚绘q‡ç»†èƒžå°ºå¯¸ç»Ÿè®¡ï¼Œç ”究人员得到hMSCã€?/span>HFFå’?/span>HUVEC¾l†èƒžçš„直径分别äØ“16.6?2.5 μmã€?/span>20.7?5.4 μmã€?/span>17.2?2.0 μm。这三ç§äººæº¾l†èƒžçš„尺寸确实å‡è½åœ¨S微井å’?/span>M微井的直径范围内åQŒå› æ­¤ä¸‰¿U类型的¾l†èƒžå?/span>能在˜q›å…¥ä¸­å¾®äº•åŽæ„Ÿåº”到适当的准三维微环境ã€?/span>
				
                  


                  
  
				
                  
					 
				
                  

                  
                  å›?/span>10. hMSCã€?/span>HFFã€?/span>HUVEC ¾l†èƒžçš„直径（æ¯ç»„n â‰?100åQ‰ã€‚图åƒæ˜¯åœ¨ç»†èƒžæŽ¥¿U到¾l„织培养æ?(TCP) 表é¢20 分钟åŽæ‹æ‘„的。所有细胞的大å°ä»‹äºŽ S å’?M 微井的直径之间ã€?/span> 该研½I?/span>设计òq¶æˆåŠŸåˆ¶å¤‡äº†å…ähœ‰å¯æ‹‰ä¼¸å¾®¾l“æž„çš„å¾®‹¹æŽ§èŠ¯ç‰‡åQŒåœ¨æ­¤åŸº¼‹€ä¸Šæž„å»ÞZº†¾l†èƒžçš„准三维é»é™„状æ€åƈ˜q›è¡Œå¾ªçŽ¯æ‹‰äŽ×刺激。细èƒ?/span>åœ?/span>ä¸åŒå¾?/span>äº?/span>阵列中的循环拉äŽ×使得¾l†èƒžå‘ˆçŽ°ä¸åŒæ°´åã^的扩散ã€?/span>å–å‘ã€è¿¿U»é€ŸçŽ‡å’Œè¿¿UÀL–¹å‘性ã€?/span>è€?/span>被èšåˆç‰©å¾®æŸ±åŒ…围的中½{‰å¾®äº•å¼•å‘了最强的¾l†èƒžå应。研½I¶äh员还研究了三¿U类型的¾l†èƒžåœ?/span>二维ã€å‡†ä¸‰ç»´å’Œä¸‰¾l´å¾®çŽ¯å¢ƒä¸­çš„表现åQ›ç»“æžœè¯å®žå‡†ä¸‰ç»´å¾®çŽ¯å¢?/span>介于二维和三¾l?/span>微环å¢?/span>åQ?/span>而且在许多方é¢ï¼Œé€‚当的准三维¾l†èƒžå¯ä»¥æ¨¡ä»¿ä¸‰ç»´å¾®çŽ¯å¢ƒä¸­çš„细胞，åŒæ—¶åˆèƒ½åƒåœ¨äºŒç»´ä¸Šä¸€æ äh–¹ä¾¿åœ°è§‚察。该研究为准三维微环å¢?/span>çš?/span>¾l†èƒžæ‹‰äŽ×æ供了有价值的工具åQŒåƈæ­ç¤ºäº†ç”Ÿç‰©æ料拓扑特å¾å¯¹¾l†èƒžçš„å¤æ‚媄å“，从而äØ“ä¸åŒ¾l´åº¦çš?/span>¾l†èƒžç ”究开辟了新途径ã€?/span> 
			
                  


                  
			
                  
				原文链接 
			
                  
			
                  
				https://www.sciencedirect.com/science/article/pii/S2452199X2100582X 
			
                  
				
                   
                  
			   
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